The inhibition folds noticed in mRNA expression were confirmed with the protein level with the assessment of TNF released into supernatants of the cultured synoviocytes (Figure 1c). Inhibition of the discharge of TNF into the supernatants with TFO at 1 nmol/l and siRNA at 75 nmol/l was respectively 62.2 2.1% and 44.2 5.8%. We assessed n . o . (NO) released into the supernatants and observed a tremendous decrease in NO secretion because of preventive transfection of both anti TNF oligonucleotides.
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